functional annotation of metagenomic contigs

Question

[serene.s]

Hi Everyone! I want to know the various tools and software that can be used to do the functional annotation of the assembled metagenomic contigs. I also want to know if I am able to understand the process correctly, according to me first step is to get the assembled contigs from short metagenomic reads (setting minimum contig length to 1000bp) then finding out the ORFs from the contigs using prokka, then get the translated protein sequences and then using some tool or database to get the functions for then translated sequences?

Thanks

[lieven.sterck]

your plan is correct.

Moreover I think that Prokka already output some sort of functional annotation as well.

Otherwise look around for papers that do similar things (eg other metagenomic papers, genome annotation papers, ...) you will get good hints from those.