minimap2 without kmer filter crashes
0
0
Entering edit mode
2.6 years ago
Martin • 0

Hello, I am trying to align pacbio hifi-reads from a dataset to the reference (in may case I use CHM13 data, but this is a general question). I use minimap2 and run following command for the alignment:

 minimap2 -c -x map-hifi -t 32 ref.mmi reads.fastq

The alignment works as intended. But now I would like to see if it makes a difference if I disable the kmer filter or just set it very low. so I run:

 minimap2 -f 0.0 -c -x map-hifi -t 32 ref_new.mmi reads.fastq

\ I experiment with "-f 0.0" an some very low values like "-f 0.00001". I found the flag in the official manual. However, minimap2 crashes for me, if I do this.

Does anyone know why that happens or does anyone have a workaround to run it without kmer filtering?

alignment minimap2 kmer • 501 views
ADD COMMENT
0
Entering edit mode

Please post the input data so we can try to reproduce it.

ADD REPLY

Login before adding your answer.

Traffic: 1801 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6