Question

How to align/ blast vector sequence to human genome

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2.6 years ago

Tonkatsu ▴ 30

Does any one have any suggestions on how to do this properly?

alignment vector Blast • 732 views

ADD COMMENT • link updated 2.6 years ago by Jeremy ▴ 930 • written 2.6 years ago by Tonkatsu ▴ 30

score 0 · Answer 1 · 2022-05-11

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2.6 years ago

Jeremy ▴ 930

You could align your vector to the human genome, but this would be a very long alignment. I would suggest using NCBI BLAST and entering Homo sapiens in the Organism field.

ADD COMMENT • link 2.6 years ago by Jeremy ▴ 930

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Thanks! So I just paste my sequence into nblast, change the organism to homo sapiens and then leave the rest of the parameters blank/default?

ADD REPLY • link 2.6 years ago by Tonkatsu ▴ 30

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That's what I would do. Let me know how it works.

ADD REPLY • link 2.6 years ago by Jeremy ▴ 930