Noted that the STAR Methods required by Cell Press require reporting the degender of cell lines used. While I order the cells from ATCC, they do not have such information available. I also tried going back to the reference/ patent/ reference, but none of them mentioned the gender/ sex of the cell line. Therefore, I am looking for a quick way to determine the sex of the cell line: using some online publicly available data.
While this question can be more general and theoretical -- if I can determine the sex of a cell line by any sequencing data and how reliable is it (given that I do not really have extra resources by doing wet-lab experiments for this authentication), I am specifically looking for if I can determine the sex of cell line by ChIP-seq data.
After downloading the ChIP peak calling output from "control" IP conditions, such as H2A, H3K4Me2, H3K27Ac. I just do a grep "chrY" Peaks.txt | head.
My rationale is that if there is any peak mapped to chromosome Y, the sample must be a male. On the other hand, if there is no peak mapped to chromosome X, the sample is highly likely to be a female (while it is not 100% certain).
May I know if this is correct? Or it would be nice if you can suggest a method that is more rigorous. Thanks!
As I am trying to get as much information as possible on the sex of cell lines in the Cellosaurus, can you tell me which mouse cell line you are using so that I can try to find out if someone somewhere has this information.