Spades assembly error

Question

[Princy]

Hello, I am using spades for WGS data, plz let me know how can i resolve these warning, I am using this script

spades.py -t 30 -1 ER2_1.fastq.gz -2 ER2_2.fastq.gz -o "/RAD_F2_186" 




======= SPAdes pipeline finished WITH WARNINGS!
=== Error correction and assembling warnings:
 * 0:05:35.557   188M / 897M  WARN    General                 (kmer_coverage_model.cpp   : 327)   Valley value was estimated improperly, reset to 1
 * 0:05:35.558   188M / 897M  WARN    General                 (kmer_coverage_model.cpp   : 366)   Failed to determine erroneous kmer threshold. Threshold set to: 1
 * 0:05:08.084   150M / 1174M WARN    General                 (kmer_coverage_model.cpp   : 327)   Valley value was estimated improperly, reset to 8
 * 0:05:08.086   150M / 1174M WARN    General                 (kmer_coverage_model.cpp   : 366)   Failed to determine erroneous kmer threshold. Threshold set to: 8
 * 0:03:14.959   194M / 1068M WARN    General                 (kmer_coverage_model.cpp   : 218)   Too many erroneous kmers, the estimates might be unreliable
 * 0:03:26.284   194M / 1068M WARN    General                 (kmer_coverage_model.cpp   : 327)   Valley value was estimated improperly, reset to 7
 * 0:03:26.285   194M / 1068M WARN    General                 (kmer_coverage_model.cpp   : 366)   Failed to determine erroneous kmer threshold. Threshold set to: 7
 * 0:05:15.442   266M / 1068M WARN    General                 (simplification.cpp        : 500)   The determined erroneous connection coverage threshold may be determined improperly

[andres.firrincieli]

google: Failed to determine erroneous kmer threshold

[Joe]

They're just warnings. You don't necessarily have to do anything about them if your output assembly looks reasonable.