qPCR Assay plot

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2.5 years ago

Maria17 ▴ 40

Dear all,

As part of my qPCR analysis, I performed two qPCR assays for the same samples using two different protocols. I have two data frames with rows of samples and columns of RNAs. The rows and columns have the same names in both data frames.

I don't know how I can compare each CT value between the two data frames shown in a barplot or other plot.

I would appreciate any solution or recommendation.

Best Regards

Maria

barplot qpcr R • 770 views

ADD COMMENT • link 2.5 years ago by Maria17 ▴ 40

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I don't think you have a clear idea of the experimental goals of your qPCR experiment so I'd recommend taking a step back. Are you trying to compare the results from the two different protocols? Are the two conditions done in a similar enough way to even compare? Are you planning on doing dCt or ddCt?

ADD REPLY • link 2.5 years ago by Trivas ★ 1.8k

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Dear Trivas,

it is the dCt, i want to compare pPCR output of two different protocol ,the samples are the same. basically comparing the number of dCt from two protocol & plotting in a bar graph or correlation graph.

Regards M

ADD REPLY • link 2.5 years ago by Maria17 ▴ 40

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