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2.4 years ago
Bio_Crap
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0
I have tried both methods with the exact same input but the # of results is different. While Geneious can generate more than 400 PCR products, the scripting method only generate 200. Both have the same setting (no mismatches allowed). I am wondering what makes the difference. Algorithm? Or others?
many things can make a difference - output may be filtered by many other parameters, melting point, distances etc
and of course it could be a flawed implementation of either - especially when you just say "Perl script" ...