Het/Mixed Indel identification tools

Question

[sangerdude]

I have sanger data (.ab1 files) that I want to call het/mixed indels on, I was wondering what are some of the tools you all use for such analysis. So far, I see Mutation Surveyor and Polyscan - I want to know what is a tool of choice? Also, what would you expect to see when comparing mixed indels between somatic and germline data?

Thanks in advance

Answer 1

[trausch]

We designed tracy to decompose het. mutations in Sanger chromatogram trace files (*.ab1). There is also a web frontend available for tracy, called indigo: https://www.gear-genomics.com/indigo/