Assembly of rna-long reads (NP)
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2.5 years ago
bioinfo_ga ▴ 70

Hi All

I want to generate consensus sequence for rna data that are generated using nanopore platform.I have tries flye and rna-bloom tools but am getting lot of contigs in rna-bloom and flye shows "No disjointigs were assembled" . Kindly suggest what could help.

Thanks
nanopore rna-seq • 694 views
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Are you getting whole genes from the RNA sequencing? How long are the contigs from rna-bloom?

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I am expecting contigs around 2-3kb but i am geeting 100bp-7kb

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