Percentage of mitochondoria in single nuclei single cellRNASeq
1
0
Entering edit mode
2.4 years ago
Tania ▴ 180

Hi All

Are these percentages of mitcohondoria seems normal for single nuclei/Single Cell RNASeq? What is a normal rate? Thanks

enter image description here

enter image description here

cell mitochondoria nuclei single RNASeq • 871 views
ADD COMMENT
2
Entering edit mode
2.4 years ago
Basti ★ 2.0k

I recommend you this paper : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599307/ Particularly : "mtDNA% ranges from 5% or less in tissues with low energy requirements up to ∼30% in the heart due to the high energy demand of cardiomyocytes" So it depends on your tissue but your percentages are in the acceptable range.

ADD COMMENT
0
Entering edit mode

Thank you Basti very much. But this is even if it is single nuclei? The paper talks about single cell? Thanks

ADD REPLY
1
Entering edit mode

You're right and I don't see any paper that studied the subject in greater depth, but I believe the percentage should be lower. An exemple here in Fig 2D : https://genomemedicine.biomedcentral.com/articles/10.1186/s13073-021-00941-8 "the percentage of mitochondrial genes sequenced in the nucleus-containing droplets was lower than the cell-containing particles (2.7% and 4.2%, respectively)" and they filtered out nuclei with >20% mitochondrial gene counts.

ADD REPLY
0
Entering edit mode

Got it. Thank you very much Basti.

ADD REPLY

Login before adding your answer.

Traffic: 1997 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6