0 enriched terms found (Gene Ontology Analysis)
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2.4 years ago
tsomakiank ▴ 50

Hello to everyone! I have the results of some RNA Seq analysis and I am trying to apply over representation analysis using clusterprofiler. I get the DEGs and then I divide them to upregulated and downregulated. Next I use the clusterprofiler to apply the overepresentation test but the result of a set of one upregulated gene set and one downregulated gene set gives back zero enriched terms. I am trying to get a goplot (goplot()) and enrichment maps (emapplot()) and in the instance of the downregulated gene set although I cant get a goplot I can get the enrichment map. My question is if that is normal sometimes and if not where i am mistaken. Also how is it possible to create the enrichment map aout of no enriched terms? Thanks in advance!

ClusterProfiler GeneOntology • 1.0k views
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How many upregulated and downregulated genes do you have?

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Hello thanks for replying. I have 172 downregulated and 216 up regulated. Note that these are from different expreimnts and not from the same.

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