How to work with .man files?

Question

[Lalit]

Hi! I want to work on a transcriptome dataset. I downloaded the dataset from NCBI database but the file has a .man extension. I do not know how to open the file. Can someone please help?

The dataset is available at https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR3147022/SRR3147022 (~15mb).

[Pierre Lindenbaum]

what is the output of file your_file.man ?

[Lalit]

Input: file Seq.man; Output: Seq.man: data

[lieven.sterck]

you will need to be a bit more specific: what kind of dataset, where did you download it from precisely, how did you download it, ... such information will only increase the usefulness of the replies you might get.

[Lalit]

The dataset is available at https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR3147022/SRR3147022

[Pierre Lindenbaum]

looks like a native NCBI SRA data. Have a look at NCBI sra-tools ?

$ wget -q -O - "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR3147022/SRR3147022" | hexdump -C | head
00000000  4e 43 42 49 2e 73 72 61  88 19 03 05 01 00 00 00  |NCBI.sra........|
00000010  bc 04 00 00 00 00 00 00  04 00 00 00 91 04 00 00  |................|
00000020  00 00 13 00 4f 00 71 00  04 00 6c 6f 63 6b f4 20  |....O.q...lock. |
00000030  b5 56 00 00 00 00 24 01  00 00 06 02 00 6d 64 f2  |.V....$......md.|
00000040  20 b5 56 00 00 00 00 6d  01 00 00 01 01 00 00 00  | .V....m........|
00000050  22 00 00 00 00 03 00 63  75 72 f2 20 b5 56 00 00  |"......cur. .V..|
00000060  00 00 24 01 00 00 02 d8  f1 00 00 00 00 00 00 7e  |..$............~|
00000070  d3 00 00 00 00 00 00 03  00 6d 64 35 f2 20 b5 56  |.........md5. .V|
00000080  00 00 00 00 24 01 00 00  02 78 00 00 00 00 00 00  |....$....x......|
00000090  00 29 00 00 00 00 00 00  00 03 00 74 62 6c f1 20  |.).........tbl. |

Answer 1

[GenoMax]

You should download this dataset (as fastq or fasta files) here: https://trace.ncbi.nlm.nih.gov/Traces/index.html?view=run_browser&page_size=10&acc=SRR3147022&display=download