How to calculate 2^-∆∆Ct correctly?

Question

[eugene]

the issue is resolved

Answer 1

[smurph50]

I found this walkthrough to be helpful

https://toptipbio.com/delta-delta-ct-pcr/#:~:text=The%20delta%2Ddelta%20Ct%20method%2C%20also%20known%20as%20the%202,(also%20known%20as%20qPCR).

[eugene]

the issue is resolved

Answer 2

[Kevin Blighe]

I provide a working example, here: How to report and plot qPCR data - delta delta Ct

Kind regards / Cordiali saluti,

Kevin