Entering edit mode
2.5 years ago
pavelasquezv
▴
50
Hi all,
When I'm running fastq-dump with some accessions everything works fine, for example:
fastq-dump --split-files SRR10345445 Results are ok
when I am running the program with other access codes I get an error, for example:
fastq-dump --split-files SRR5631167
connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -9984 ( X509 - Certificate verification failed, e.g. CRL, CA or signature check failed )
2022-06-15T18:43:42 fastq-dump.2.9.6 sys: mbedtls_ssl_get_verify_result returned 0x8 ( !! The certificate is not correctly signed by the trusted CA )
2022-06-15T18:43:42 fastq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '52.217.164.177' from '143.107.211.130'
2022-06-15T18:43:42 fastq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-pub-run-odp.s3.amazonaws.com' (52.217.164.177) from '143.107.211.130''
This error only happens when I run the program on the server, because from my pc everything works fine.
Please, does anyone know how to fix this problem? I have many sequences to download :(
Many thanks
sounds like the security certificate handed out by your server is not valid (or at least not signed or recognized).
I think your best option is to contact your server admin and see what you need/can do.
There are another way to make download of the runs by the server?
you can try going via ENA?
you can use this webtool to get download links (several ways) : sra-explorer.info
(perhaps you can also get alternative ways to download from SRA)
Many thanks for you reply. I will try!
Best wishes!
Hi, could you please let me know how to upgrade sratoolkit in centos? Thanks and regards,