I am currently trying out different transformation and am visualizing the resulting differences in a UMAP/TSNE. It would be very helpful, to see how the clusters (i.e. cells) change before or after the transformation.
I imagine something like a faint line between the same cell in the UMAP before and after the transformation. I have included a sample image to illustrate what I mean.
Is anybody aware of a package/function/ shinyApp that does something similar to this?
Or for example you select cells in one embedding and they light up on the different embedding (after transformation)
Any help is much appreciated!
Cheers
What does transformation mean here? The mathematical operation or the biologial process?
Mathematical operation. Its actually the
multiBatchNorm
function, so adjusting thesizeFactor
for the different experimental batches.The picture that I have added is not my actual data by the way...