sequence error

Question

[maricom]

Hi,

What kind of base sequence errors could happen more frequently at MiSeq? I found a document saying G > A and C > T could occur more, but why?

Thank you.

Answer 1

[mark.ziemann]

Those base mutations are called transitions. Purine-> Purine or Pyrimidine -> Pyrimidine. Transversions are when a purine is mutated to pyrimidine or vice-versa. Transitions are more common in nature and in PCR. Some related links:

1. https://en.wikipedia.org/wiki/Transversion
2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720931/
3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5026502/

[maricom]

Thank you, Mark!!