Single cell

Question

[shiviagarwalpharma]

HI Guys,

I am analyzing a single cell data containing 22815 features (genes) across 1347 samples (cells) within 1 assay. I am following the Seurat tutorial (https://satijalab.org/seurat/articles/pbmc3k_tutorial.html) and able to calculate variable features (genes) and principal components. I also tried clustering them using UMAP. My queries are:

1. These variable features and principal components are computed based on feature data coming from all 1347 cells. I mean they are kind of averaged from all cells. Please confirm if I understand correctly.
2. I want to have list of DE or variable feature from each cell. How can I do this ?
3. I want to distinguish the cells having gene signature 1 and the cells having gene signature 2. How can I do this ? I already have a list of gene signature 1 and gene signature 2, and want to map them with each cell variable feature. Please give your suggestions.

Thanks in advance