Entering edit mode
2.3 years ago
m.radz
▴
10
I have short read sequences of several isolates that I want to assemble into contigs. I know what these isolates are so I can use a reference genome to improve the assembly process.
Trouble is I don't know what assembler to use, I typically work with metagenomes so use tools like spades and megahit, although to my understanding they don't use a reference file.
What is the alignment fraction and ANI of the draft assemblies compared to the reference genomes?