What are some Prokayotic assembly tools that use a reference fasta
0
0
Entering edit mode
2.3 years ago
m.radz ▴ 10

I have short read sequences of several isolates that I want to assemble into contigs. I know what these isolates are so I can use a reference genome to improve the assembly process.

Trouble is I don't know what assembler to use, I typically work with metagenomes so use tools like spades and megahit, although to my understanding they don't use a reference file.

Genome Assembly Prokaryotic • 520 views
ADD COMMENT
0
Entering edit mode

I know what these isolates are so I can use a reference genome to improve the assembly process

What is the alignment fraction and ANI of the draft assemblies compared to the reference genomes?

ADD REPLY

Login before adding your answer.

Traffic: 1741 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6