Entering edit mode
2.4 years ago
Maka
▴
20
hi...I am trying to run the Chip-Seq analysis pipeline...
bwa mem2 for reference genome GRCm39 download from https://www.ncbi.nlm.nih.gov/assembly/GCF_000001635.27 as .fna (genbank)
#index genome
cd /media/j/Data/BWA-MEM2
bwa-mem2-2.0pre2_x64-linux/bwa-mem2 index "/media/j/Data/reference_genome/GCA_000001635.9_GRCm39_genomic.fna"
output
[bwa_index] Pack FASTA... 6.45 sec
init ticks = 204276126157
ref seq len = 5456444902
binary seq ticks = 108645462914
build index ticks = 4181642648109
ref_seq_len = 5456444902
count = 0, 1585357357, 2728222451, 3871087545, 5456444902
BWT[2307856312] = 4
CP_SHIFT = 5, CP_MASK = 31
sizeof CP_OCC = 64
Killed
As far as I understood, it is a problem of memory, but I checked and should have enough space (8T)
Any suggestions? thanks in advance!
Disk space is not memory, and no or barely any machine in the world has 8T of memory. Type
free -hm
to learn how much memory you have.sorry, I misunderstood! so I checked it and it is a total mem 62G...
you have 8Tb of memory in your server? I think you might be confusing it with storage/disk space .
can you add/post the specs of the server/laptop/pc you are trying to run this on?