Entering edit mode
2.2 years ago
Chris
▴
340
Hello all,
Would you please explain what leaky RNA means? Google didn't know this.
Hello all,
Would you please explain what leaky RNA means? Google didn't know this.
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Oh. Sorry about that. I thought this concept can understand without context. It is from a course in scRNA-Seq using 10x technique (droplet-based, can have leaky RNA). Your comment is interesting and if the image is larger, it will be easier to read the keyword.
Never heard of it. Maybe ambient RNA contamination is what they mean.
Thank you! English is not the first language of the lecturer, so maybe the word choice is not clear. Would you please explain ambient RNA? I looked on the internet but still not clear. Where does ambient RNA come from?
Droplets that don't end up with a cell inside of them could potentially acquire reads from extracellular RNA (e.g. from lysed/broken cells, etc...) is probably what they meant. It's essentially false positive signal.
Some examples here:
Inhibition of human immunodeficiency virus type 1 by RNA interference using long-hairpin RNA
RNA Polymerase-specific Nucleosome Disruption by Transcription in Vivo
The RBPome: where the brains meet the brawn
Thank you so much!