Question

mapping paired end data

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2.3 years ago

upasana ▴ 20

Hello folks,

Can I map paired end data individually with my reference genome to get separate .bam files?

paired-end bam bowtie mapping • 884 views

ADD COMMENT • link updated 20 months ago by Ram 44k • written 2.3 years ago by upasana ▴ 20

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Yes. Just map each one individually (I would not recommend it but it's possible)

ADD REPLY • link 2.3 years ago by Asaf 10k

score 1 · Answer 1 · 2022-08-22

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2.3 years ago

swbarnes2 14k

Sure, but why not use the information from the one member of the pair to help map the other? You can map them together, then filter the bam to pull out the all the read1 reads.

ADD COMMENT • link 2.3 years ago by swbarnes2 14k

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okay.. got it.. thanks much:)

ADD REPLY • link 2.3 years ago by upasana ▴ 20