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2.2 years ago
ummamd
•
0
Hey, I have sequenced a recombinant plasmid and I am trying to characterize the insert sequences that have not aligned to the reference. I have generated a BAM file with the unmapped sequences and now I am trying to BLAST these in various databases to identify the unmapped sequences.
What is your question?
Assuming you are studying microbes. Maybe you can try de novo assembly and blast the contig to NCBI or other public database.
Velvetassembler source code: https://github.com/dzerbino/velvet