Why figure from guppy basecaller show only 1 base in each event?
1
0
Entering edit mode
2.2 years ago

Hi, I am a beginner in nanopore. So I study about the guppy basecaller and try to use it. In normally from each peak in raw signal that represent current for each 5-mer. I wonder why in this picture that show the only 1 base for each peak instead show AACAT, CCGGT?

enter image description here

basecally guppy nanopore • 732 views
ADD COMMENT
0
Entering edit mode

Probably because they wanted to keep the representation simple and are just showing the consensus calls on one strand.

ADD REPLY
0
Entering edit mode
14 months ago
Leon ▴ 130

briefly,it use middle base to represent 5mer, similarly in tombo resquiggle

ADD COMMENT

Login before adding your answer.

Traffic: 1836 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6