How can the reads be assemble to unitigs with ONLY EXACT sequence overlaps?
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2.2 years ago
BioGeek ▴ 170

I'm now working on a metagenome assembly project. There, I want to segregate and assemble all of the reads with stringent parameters: they should not overlap segments even if they have a single mutation in the sequence. Is there any software/tools or flags that can assist me in accomplishing this...

Note: At the moment I don't care about N50.

Genome Assembly • 517 views
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2.2 years ago
shelkmike ★ 1.4k

Not 100% sure, but probably unitigs of BCALM are what you need (https://github.com/GATB/bcalm)

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