Entering edit mode
2.2 years ago
noobiestar
▴
30
Hi, I am using qiime2 v 2021.2 for 16s rrna analysis. I am planning to train naive bayes classifier for taxonomic assignment. For this I am using green gene 13_8. On importing otu.fasta file using the command: qiime tools import \ --type 'FeatureData[Sequence]' \ --input-path greengenes/97_otus.fasta \ --output-path greengenes_97_otus.qza I am gettiing error as this: Invalid character '-' at position 0 on line 2 (does not match IUPAC characters for this sequence type). Allowed characters are ACGTURYKMSWBDHVN. I checked the otu.fasta file and it is having many '_'. How to import the otu.fasta file in qiime2?
