Are there any experiments to detect "O6 Guanine methylation levels" ? If so what are they?

(I am not asking about the Methylspecific PCR or Pyrosequencing or IHC or WB for MGMT gene, but a way to determine the levels of O6 guanine methylation levels ?)

Thanks in advance!

Detection NGS O6 methods levels methylation assays experiments Guanine • 589 views

ADD COMMENT • link 2.2 years ago by Guruprasadh ▴ 20

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Might be worth talking to Oxford Nanopore? I know that they can already detect 5mC, 5hmC, 6mA, and BrdU in DNA and m6A in RNA and might be interested to expand that set if there is a reasonable avenue (known molecules to train the algorithms on and commercial interest).

ADD REPLY • link 2.2 years ago by Matthias Zepper 5.0k

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Dear Matthias,

Thanks for the inputs! Yes a good suggestion indeed. Thanks

ADD REPLY • link 2.2 years ago by Guruprasadh ▴ 20

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