DADA2 or Deblur

Question

[amaddheshiya1610]

Hello Everyone,

I am a Ph.D. student working in the area of child health and microbiome. I have done 16S amplicon sequencing using NovaSeq 6000. I'm using QIIME2 for sequence analysis. I have used DADA2 as well as Deblur for denoising but DADA2 takes too much time (it took 3 days for 4 samples) as compared to Deblur. my question is which one is better?

Thank you very much, any help will be very appreciated!

Best regards Ankita

[Arup Ghosh]

DADA2 vs DEBLUR

https://peerj.com/articles/5364/

https://www.nicholas-ollberding.com/post/denoising-amplicon-sequence-variants-using-dada2-deblur-and-unoise3-with-qiime2/

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227434&type=printable