CreatSeuratObject command issue

Question

[sandy]

Hello everyone,

I am trying to create an object on R, however the system says

Error in CreatSeuratObject(counts = p1, min.features = 200, min.cells = 3, : could not find function "CreatSeuratObject".

My code is:

p2<- CreateSeuratObject(counts = p2, project = "panc_adj", min.cells = 3, min.features = 200)

Session info: locale: [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C

attached base packages: [1] stats graphics grDevices utils datasets methods base

other attached packages: [1] ggthemes_4.2.4 RColorBrewer_1.1-3 patchwork_1.1.2 ggplot2_3.3.6
[5] Matrix_1.5-1 cowplot_1.1.1 sp_1.5-0 SeuratObject_4.1.2 [9] Seurat_4.2.0 dplyr_1.0.10 readr_2.1.3

loaded via a namespace (and not attached): [1] Rtsne_0.16 colorspace_2.0-3 deldir_1.0-6
[4] ellipsis_0.3.2 ggridges_0.5.4 spatstat.data_3.0-0
[7] leiden_0.4.3 listenv_0.8.0 ggrepel_0.9.1
[10] bit64_4.0.5 fansi_1.0.3 codetools_0.2-18
[13] splines_4.0.5 polyclip_1.10-4 jsonlite_1.8.3
[16] ica_1.0-3 cluster_2.1.3 png_0.1-7
[19] rgeos_0.5-9 uwot_0.1.14 shiny_1.7.3
[22] sctransform_0.3.5 spatstat.sparse_3.0-0 compiler_4.0.5
[25] httr_1.4.4 fastmap_1.1.0 lazyeval_0.2.2
[28] cli_3.4.1 later_1.3.0 htmltools_0.5.3
[31] tools_4.0.5 igraph_1.3.5 gtable_0.3.1
[34] glue_1.6.2 RANN_2.6.1 reshape2_1.4.4
[37] Rcpp_1.0.9 scattermore_0.8 vctrs_0.5.0
[40] nlme_3.1-159 progressr_0.11.0 lmtest_0.9-40
[43] spatstat.random_2.2-0 stringr_1.4.1 globals_0.16.1
[46] mime_0.12 miniUI_0.1.1.1 lifecycle_1.0.3
[49] irlba_2.3.5.1 goftest_1.2-3 future_1.28.0
[52] MASS_7.3-58.1 zoo_1.8-11 scales_1.2.1
[55] spatstat.core_2.4-4 vroom_1.6.0 hms_1.1.2
[58] promises_1.2.0.1 spatstat.utils_3.0-1 parallel_4.0.5
[61] reticulate_1.26 pbapply_1.5-0 gridExtra_2.3
[64] rpart_4.1.16 stringi_1.7.8 rlang_1.0.6
[67] pkgconfig_2.0.3 matrixStats_0.62.0 lattice_0.20-45
[70] ROCR_1.0-11 purrr_0.3.5 tensor_1.5
[73] htmlwidgets_1.5.4 bit_4.0.4 tidyselect_1.2.0
[76] parallelly_1.32.1 RcppAnnoy_0.0.20 plyr_1.8.7
[79] magrittr_2.0.3 R6_2.5.1 generics_0.1.3
[82] pillar_1.8.1 withr_2.5.0 mgcv_1.8-40
[85] fitdistrplus_1.1-8 survival_3.4-0 abind_1.4-5
[88] tibble_3.1.8 future.apply_1.9.1 crayon_1.5.2
[91] KernSmooth_2.23-20 utf8_1.2.2 spatstat.geom_3.0-3
[94] plotly_4.10.0 tzdb_0.3.0 grid_4.0.5
[97] data.table_1.14.4 digest_0.6.30 xtable_1.8-4
[100] tidyr_1.2.1 httpuv_1.6.6 munsell_0.5.0
[103] viridisLite_0.4.1

Answer 1

[ATpoint]

Learn to read the error: CreatSeuratObject => there is an "e" missing, CreateSeuratObject

[sandy]

I think I spell it correct. My code is p2 <- CreateSeuratObject(counts = p2, min.features = 200, min.cells = 3, project='panc_adj').

[ATpoint]

???

however the system says Error in CreatSeuratObject(counts = p1, min.features = 200, min.cells = 3, : could not find function "CreatSeuratObject".

[sandy]

Ok. I forget to change this. But I indeed might a different error with correct spelling.