how to decide the strandness of the RNAseq data
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2.3 years ago
dew ▴ 10
https://rseqc.sourceforge.net/#infer-experiment-py

Does the result Fraction of reads explained by "1+-,1-+,2++,2--": 0.9441 means --rf-stranded ?

Thanks a lot!

strandness RNA-Seq • 659 views
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2.3 years ago
iraun 6.2k

yes, reverse strand specific.

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Thanks a lot!

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