Meta-analysis using 16S rRNA NGS data
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23 months ago
bala • 0

Hi everyone

I am running a meta-analysis using 16S rRNA NGS data, most of the file have not primer information in their corresponding papers.

Kindly suggest me is their any tool that automatically detect and remove 16S primer from the sequence.

By the way i am using DADA2 for downstream process, (filterandtrim can be used to remove primer by trim =c(19,17 )) But, I want to remove primer before DADA2.

I have tried with rbind option with known V4 primers, but it's not working.

Thank you for your time and suggestion

Primer • 702 views
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Entering edit mode
23 months ago

Use a regular filter such as Cutadapt, Trimmomatic, Trim-Galore or the like to this end

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