At what threshold should module eigengenes needs to be cut to merge modules after dynamic tree cut?
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23 months ago

Hello,

I have performed WGCNA on my mRNA expression dataset containing 709 genes and 10 samples. After dynamic tree cut I got 10 modules (including the grey module). However, I tried to merged the modules with similar expressions, I only got 3 modules (pink, brown, and black) at the ME dissThreshold value of 0.25. Should I proceed with these 3 modules or should I change the threshold value?

I have tried changing the values of MEdissThres, at 0.20 = same 3 modules at 0.10 = only my blue and red modules merged otherwise all other modules remain the same.

for your consideration, I have provided the following image files.

cluster tree

dendrogram

WGCNA • 619 views
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