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2.4 years ago
sy20213040737
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0
I am using maker to annotation a genome. Result file (gff3 format) from Round1 and Round2 can found genes. Round 1 result file can be searched 17295 genes. Round2 can be searched 17000+ genes,too. But round 3 is 0 gene. I less the gff3 file manually, column 3 are all contigs, there is no gene, no CDs, no exon, no mRNA.
I put the round2 result in to ronud3, the different options from round2 are est_gff, protein_gff, rm_gff, the rest are all the same.
What the problem is?