Metagenome analysis on EPI2ME ONT
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2.0 years ago

Hello! We sequenced mosquitoes gut using SQK-16S024 kit on MIN106 flowcell. Mean Q-score is about 11, Mode Q-score is about 10. Now we want to analyse bacterial composition in mosquitoes using EPI2ME Fastq 16S tool. I want to know the following: Fastq 16S uses BLAST for compositional analysis therefore if you were to set some BLAST metrics on your own, which percentage of the BLAST identity (which is 77% by default) would be acceptable based on our Q-score? Are there any suggestions? I appreciate it in advance!

metagenomics ONT 16S longreads • 553 views
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