Extract alignment in fasta format from BAM/SAM file
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Entering edit mode
22 months ago
SaltedPork ▴ 170

Hi, I have a BAM file from aligning reads to a reference. What I would like is a fasta alignment file for each read showing the alignment between read and reference ie.

>read1

------A--C------GTATCTAGTCATGACT----------

Is there a tool/method that I can use that can extract the alignmments from a BAM and show in fasta format.

alignment bam • 1.1k views
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2
Entering edit mode
22 months ago

The tool sam2pairwise does what you need:

https://github.com/mlafave/sam2pairwise

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