Entering edit mode
22 months ago
sunyeping
▴
110
Dear all,
I am trying to construct a plasmid vector that contains a vaccinia virus protomer (p7.5, p11, etc.) and a marker gene (GFP, mcherry, etc.). I can find the sequences of the promoter. For example, the sequence of the p7.5 is
5'-TAAAAGTAGAAAATATATTCTAATTTATTGCAC-3’[J. Mol. Biol. (1989) 210, 749-769]
but what should be the length and the sequence of the marker gene?
Thank you in advance!