Entering edit mode
22 months ago
fr
▴
220
Hi,
I'm confused by the StringTie calculation of TPM (docs) for output TPM. They point to this paper for the definition of TPM (but I can't find much about it), and to this tutorial for explaining what TPM is. However I find that some genes with 0 counts show a positive very low TPM (0.15). Can someone please explain how this can be so given that the following is the calculation of TPM:
TPM = ( counts * sum_counts_in_sample) / ( gene_length_kb * 1e6)
Thanks in advance