blast engine error empty blast query vector

Question

[Sowmya Pulapet]

Hi,

I am trying to align a nucleotide sequence against a reference genome using blastn command line version. The command I used was:

blastn -query file.fa -subject genome.fasta -out blast.txt -outfmt "6 sseqid sstart send sseq pident"

I got the error:

blast engine error : Empty CBlastqueryvector

My query file is not empty and there are no duplicate headers. Also there are no special characters or symbols.

Please help me to solve this . Thanks in advance!

[GenoMax]

Which version of blast are you using?

[Sowmya Pulapet]

blast 2.13.0 installed using anaconda.

[pbioinf]

What is the output of head file.fa ?

[e.r.zakiev]

same problem here