Analyzing bulk RNA-seq

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22 months ago

Zaid • 0

Hi all, I have a bulk RNAseq dataset for a cell line and want to analyze it using single-cell analyses. Is there any package I could use on R that could do that?

Thanks,

RNAseq • 722 views

ADD COMMENT • link updated 22 months ago by swbarnes2 14k • written 22 months ago by Zaid • 0

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A bulk RNA Seq data set is not a single cell RNA data set, and there is no R package that can convert bulk data to single cell data. If you want a single cell data set, you have to do a single cell experiment. Is this a useful activity for a cell line? What are you actually trying to acheive?

ADD REPLY • link 22 months ago by seidel 11k

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To paraphrase another poster here, you are asking how to get a cow out of a steak. You can't do it. A bulk RNASeq experiment does not contain the information about which read came from which cell. No R program can a magically create that information.

ADD REPLY • link 22 months ago by swbarnes2 14k

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