exchange the monocle data with Seurat

Question

[alghezim1]

Hi everyone! I am pretty new to single cell RNA seq analysis. I am using monocle 3 for my analysis. the data file that I have downloaded has already normalized data and it was coming with ensembl ID and I was able to get the gene name from monocle but I was wondering if there is a way to shift the information from monocle to Seurat to do do my pre-processing steps (QC) and UMAPS

thank you!

Answer 1

[fracarb8]

exportCDS(monocle_object, export_to = 'Seurat', export_all = T)

Alternatively, you can directly use seurat with your normalise data. In that case, based of the type of pre-processing that was done, you need to skip the normalisation-scaling steps.

[alghezim1]

Hi there,

I am getting this error when running the code Error in exportCDS(monocle_object, export_to = "Seurat", export_all = T) : could not find function "exportCDS"