pileup consolidation tool
0
0
Entering edit mode
21 months ago
noodle ▴ 590

Hello Biostars,

I'm looking for a pileup tool that will consolidate output to a table that is strand sensitive, separates +/-, includes indels as well count of starts and stops. I'm familiar with many tools, but it seems like none of them are able to capture everything listed. JACUSA2 is the closest I've found but this doesn't have al the features. I've included an example header below for what I'm after. Thanks!

chr loc ref strand  A   T   C   G   a   t   c   g   Insertion   Deletion    Stops   Starts
1   888659  T   +   0   9   0   2   0   0   0   0   2:AG|1:AGAG 1:ATT   3   2
RNAseq pileup jacusa samtools • 703 views
ADD COMMENT
0
Entering edit mode

what is the scientific question you're trying to answer ?

ADD REPLY
0
Entering edit mode

Detection of RNA modifications.

ADD REPLY
0
Entering edit mode

I tried to use Rsamtools::pileup() but haven't been able to come up with the right parameters to pass in PileupParam ...maybe somewhere here knows better?

ADD REPLY

Login before adding your answer.

Traffic: 1867 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6