Using DESeq2 to data normalization

Question

[Hello]

Hello,

I have a single question in DESeq2 usage. I have multiple groups, but each has a single sample. Therefore, I could not use DESeq2 to obtain the fold change. Alternatively, I have a plan to do GSEA, but GSEA requested a normalized data as an input.

In that case, could I use DESeq2 only for normalisation? It looked ok but I want to make it sure.

Thank you very much.

[tothepoint]

Yes, you can technically use DESeq2 as it performs normalization to adjust for differences in sequencing depth between samples.

[Hello]

Thank you very much.