differential expression analysis

Question

[Vikram]

Hello! How to perform differential expression analysis of RT-PCR data for two conditions say control and treatment?

[Kevin Blighe]

Please confirm the processing that has already been performed on the data. If you are not aware of any, then please ask your supervisor, colleague, or service provider about this, and then get back to us.

Answer 1

[bkleiboeker]

The deltaDeltaCt method is most common. It's usually appropriate to conduct statistics on deltaCt values, and present RQ (2^-ddCt). Google more for yourself, or see

https://toptipbio.com/delta-delta-ct-pcr/