MGI Sequencing - Best Script for fixing FASTQ file headers coming from MGI Sequencer's

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21 months ago

Waly • 0

Any suggestions for a good script for fixing the headers for FASTQ files coming out of MGI Sequencers?

NGS • 1.2k views

ADD COMMENT • link updated 21 months ago by GenoMax 147k • written 21 months ago by Waly • 0

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Curious as to why they need "fixing"? Can you show examples of what needs to be fixed.

ADD REPLY • link 21 months ago by GenoMax 147k

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Can I use them directly for alignment after QC, and recommendations for Alignment scripts that take these FASTQ files, I'm just very new to their analysis? I used to QC FastQ from Illumina and use for Botwei alignment for example, and I'm not sure if I may need to fix headers for MGI FASTQ files .. can you offer me an advice?

ADD REPLY • link 21 months ago by Waly • 0

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If you were able to use FastQC and bowtie for alignment then no "fixing" should be needed for MGI headers. Since MGI seq is not common in US show us examples of what the headers look like.

ADD REPLY • link 21 months ago by GenoMax 147k

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