Gene Enrichment analysis with EggNog output

Question

[JirMan]

Hi everyone!

I want to perform a gene set enrichment analysis of differently expressed host genes during virus infection. I performed Gene Ontology (GO) annotation with eggnog for the whole host genome and got one .tsv file, an excel file, and two .orthologs files. I've been trying to find literature on how to continue and how to use those files as input for the next tool (can someone recommend a good R package that is compatible with eggnog?), but without success.

I'm very new to this, is there anyone who could give me a hint on how to continue or maybe a protocol/paper that describes the downstream processes after the eggnog annotation?

Please let me know if you need more specific information, any help is highly appreciated!

Thank you!

[F.Bedjou]

I am new with Bioinformatic so I've been going around in circles for a few months, analyzing my eggnog results I have a transcriptome and candidate genes I want to do an enrichment test between them, draw graphs, observe the pathway maps and understand the results to which each COG, GO, KO, EC number.... etc. corresponds;

example:

KEGG_ko

kb:K12979
kb:K10603
-
-
kb:K12385

I found an article that talks about TBtools , there is an Eggnog-mapper helper part which is supposed to give separate files for each column with the most important code but unfortunately it does not work with me, maybe that will help you ..

[JirMan]

Thank you for taking the time to respond to my issue. Unfortunately, your approach only confuses me the more, my apologies ... there must be a more simple way to use the tsv file which contains my DEGs of interest for GSEA, but I already saw several other forum threads with a similar question to mine but none of them really resulted in a helpful answer...

Answer 1

[mr.randolph85]

I found this blog post that walks you through it.

https://archetypalecology.wordpress.com/2021/01/27/how-to-perform-kegg-and-go-enrichment-analysis-of-non-model-species-using-r/