SRA prefetch error

Question

[Foad]

I am using sratoolkit.3.0.2.ubuntu64. I tried downloading RNA-seq data with the command prefetch ERR315616 or any other data (ERR).

I got the error message . This process takes a lot of time and I didn't face this problem but now i have.

foad@Linux:~$ sratoolkit.3.0.2-ubuntu64/bin/prefetch -p srr030252

2023-03-13T11:57:29 prefetch.3.0.2: Current preference is set to retrieve SRA Normalized Format files with full base quality scores.
2023-03-13T12:07:33 prefetch.3.0.2 int: connection failed while opening file within cryptographic module - cannot open remote file: https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR030252/SRR030252
023-03-13T12:19:29 prefetch.3.0.2: 1) Downloading 'SRR030252'...
2023-03-13T12:19:29 prefetch.3.0.2: SRA Normalized Format file is being retrieved, if this is different from your preference, it may be due to current file availability.
2023-03-13T12:19:29 prefetch.3.0.2:  Downloading via HTTPS...
2023-03-13T12:45:03 prefetch.3.0.2 int: connection busy while validating within network system module - Cannot KNSManagerMakeClientRequest: https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR030252/SRR030252
2023-03-13T12:45:03 prefetch.3.0.2: 1) failed to download 'SRR030252': RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy)

Please help and thanks in advance.

[GenoMax]

Can download ERR315616 using prefetch. So this may be some local issue. Especially if you are behind a firewall.

[dthorbur]

I have the same problem. sratoolkit doesn't work on the university HPC. An alternative is using wget and getting the ftp address from the ENA browser. Just download the report in tsv format and it'll have the full ftp link for all selected samples.