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20 months ago
theadrijasaha
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What's denoted by two separate given R1 and R2 files while downloading Submitted Files under each Study Accession from ENA?
What's denoted by two separate given R1 and R2 files while downloading Submitted Files under each Study Accession from ENA?
This denotes paired-end sequencing data.
See for explanation:
Single-end sequencing versus paired-end
Some explanation about what a paired-end sequencing really means
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Are these the raw files directly submitted to ENA?
Almost. The read names are simplified and the technical reads (like spike ins) should be filtered out. You may find library construction adapters or Illumina primers in some data sets at least.