Entering edit mode
20 months ago
hellokwmin
•
0
Hello, Biostars,
I am trying to perform GO analysis for transcriptome of non-model organims (i.e., one of moss species). So far, I have done DESeq2 analysis.
Now, I have files in order to try GO analysis as follows:
- DESeq output file (i.e. control vs. treatment)
- Diamond blastp output file (tabular m.8 file); this file shows accession id corresponding to my query sequence such as XP_021739596.1. and also other information as follows: query seq-id, subject seq-id, percentage of identical matches, alignment length, number of mismatches, bit score etc.
There are so many tools for GO analysis such as Bingo, R-related packages, but quite difficult to determine which one is the best choice in my case using TWO files as described above...
Do I need to convert accession id to gene_id for further analysis? Is there any tutorial that I can follow in relation to my situtation?
Help me plz...