Entering edit mode
20 months ago
Confused_human
▴
30
Hi everyone,
I have a dataset that has 1000 gene code ids that I got from RNA sequencing. I want to convert the gene code ids to ensemble id or entrez id so that I can do its functional enrichment study to find apoptotic gene from the list of genes.
Example of gene code Ids -
ENSG00000221746.1
ENSG00000279324.1
ENSG00000233595.2
ENSG00000162374.14
ENSG00000230828.1
ENSG00000237337.1
ERCC-00165
ERCC-00168
ERCC-00170
ERCC-00171
Please suggest me a tool or command that can do this work.
Thank you
These are already Ensembl gene ID's (
ENSG*
).ERCC
id's are spike-in controls. Perhaps you want to get gene ID's from these" : ENSEMBL Gene ID to Gene Symbolsmall thing: i.e. ENSG00000162374.14 is a stable ENSEMBL ID plus version. But the gene versions may be specific to a given ENSEMBL release, so the aforementioned ID will not be found in ENSEMBL 109 Biomart (the gene id is now: ENSG00000162374.18). You may strip .14 from the ID (easiest), but this obscures the info that your read counts are from older annotation release.
Hello thank you so much for the reply . I want to find the function of these genes . When putting them into David d software I am getting 70-80% results, for rest I'm not getting any GO assigned to the ID . Any idea which tool will work better? And also I'm new to this RNA seq thing so can you please explain do I need to find function for the ERCC ID also ?