How to know the sample total read in SRA

Question

[m90]

Hello there,

I have a project that I should select sample that has from 25 to 50 million read how I know that? And to someone explain to me what spot and bases mean? When say the run SRR34558 has 28.8 M and bases 3.6 Gbp what I should understand from those numbers?

[Ram]

Have you tried Googling these terms? Where exactly did you run into difficulties understanding them

[m90]

Not exactly about the terms, it’s about that I should find sample with average 25 to 50M reads so I was wondering if the spot number is actually the total number of reads in the sample or where exactly I should search for it to know?

Answer 1

[GenoMax]

Concept of a spot is probably deprecated at this time but it used to mean this in days past (What Is A "Spot" In Sra Format ).

For current data those numbers should mean what they say. There are NN million reads adding up to NN gigabases.

[m90]

So what you mean that the total number of spots is total number of reads right?

[Ram]

Yes, that's what he's saying. It's NN million reads or pairs of reads depending on SE/PE protocol. I just verified it using a sample I uploaded for my lab.