Entering edit mode
18 months ago
ebogen
•
0
Hi everyone, I am trying to use trimmomatic on a few RNAseq files but I keep getting the error below. I have tried changing the path and checked for any white spaces but I keep getting the same error.
java -jar /packages/7x/trimmomatic/0.33/trimmomatic.jar PE OBG0044-1-010_S37_L005_R1_001.fastq OBG0044-1-010_S37_L005_R2_001.fastq OBG0044-1_R1.paired.trim.fastq OBG0044-1_R1.unpaired.trim.fastq OBG0044-1_R2.paired.trim.fastq OBG0044-1_R2.unpaired.trim.fastq ILLUMINACLIP:adapter_other.fa SLIDINGWINDOW:4:30
Error
Exception in thread "main" java.lang.ArrayIndexOutOfBoundsException: Index 1 out of bounds for length 1
at org.usadellab.trimmomatic.trim.IlluminaClippingTrimmer.makeIlluminaClippingTrimmer(IlluminaClippingTrimmer.java:53)
at org.usadellab.trimmomatic.trim.TrimmerFactory.makeTrimmer(TrimmerFactory.java:27)
at org.usadellab.trimmomatic.TrimmomaticPE.run(TrimmomaticPE.java:496)
at org.usadellab.trimmomatic.Trimmomatic.main(Trimmomatic.java:35)
Adapter file
>TruSeq_Universal_Adapter
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
>TruSeq_Adapter_Index 1-64
GATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG
Is
adapter_other.fa
the name of this adapter file (one that you show above)? That is what you have in your command line.yep, that is the one refed in the command line